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17 June 2025
| 08:00 – 09:00 | Registration |
| Chair: Anna Aknamova | |
| 09:00 – 09:20 | Opening: – Imagine! by Anna Akhmanova – NL-BioImaging by Eric Reits – 3D-Nanoscale Imaging by Sjoerd Stallinga – NVvM by Ben Giepmans |
| 09:20 – 10:00 | Imaging the Molecular Processes of Cell Division Across Scales Jan Ellenberg |
| 10.00 – 10.25 | Organoid human cancer immunity model in motion Anne Rios |
| 10:25 – 10:55 | Coffee break |
| Chair: Ben Giepmans | |
| 10:55 – 11:20 | Zooming in and out of cellular ultrastructure with fast 3D electron microscopy Jacob Hoogenboom |
| 11.20 – 11.35 | High-throughput single molecule microscopy with adaptable spatial resolution using exchangeable oligonucleotide labels Ran Huo |
| 11.35 – 11.50 | Enabling autonomous, high-throughput single-molecule microscopy Koen Martens |
| 11:50 – 12:30 | Scaling Life: How Single Cells Orchestrate Tissue-Level Coordination Prisca Liberali |
| 12:30 – 13:30 | Lunch |
| 13:30 – 15:30 | Poster session I |
| 15:30 – 16:00 | Tea break |
| Chair: Lukas Kaptein | |
| 16:00 – 16:15 | Enlightening the Role of Microtubules in Mesenchymal Migration Joyce Meiring |
| 16:15 – 16:30 | 3D Spatial Organization and Dynamics of Dendritic Cell Podosomes on Decellularized Dermal Matrices Koen van den Dries |
| 16:30 – 17:10 | Imaging the Evolutionary Journey of Centrosomes and Cilia Monica Bettencourt-Dias |
| 17:10 – 17.25 | Closing words |
| 17:25 – 18:25 | Drinks |
18 June 2025
| 09:00 – 09:05 | Opening |
| Chair: Sjoerd Stallinga | |
| 09:05 – 09:45 | Adding dimension to intravital Imaging Scott Fraser |
| 09.45 – 10:00 | Towards data-driven segmentation of cellular ultrastructure through hyperspectral electron microscopy Peter Duinkerken |
| 10.00 – 10.15 | Dissecting the cell type-specific subcellular organization in intestinal organoids using Expansion Microscopy Thijs Makaske |
| 10:15 – 10:45 | Coffee break |
| 10:45 – 12:45 | Workshop: NL-BioImaging Workshop: 3D-Nanoscale Imaging |
| 12:45 – 13:45 | Lunch |
| 13:45 – 14:45 | Poster session II |
| 14:45 – 15:15 | Tea break |
| Chair: Eric Reits | |
| 15:15 – 15:30 | NanoBlocks: creating fluorescent biosensors from affinity binders using competitive binding Silke Denis |
| 15:30 – 15:45 | Multispectral live-cell imaging at uncompromised spatiotemporal resolution Akaash Kumar |
| 15:45 – 16:25 | Exploring life’s structures across scales through imaging Jennifer Lippincott-Schwartz |
| 16:25 – 16:40 | Closing words |
| 16:40 – 17:40 | Drinks |